AKTIVITAS ANTIBAKTERI ACTINOMYCETES DARI AKAR TANAMAN DI EKOSISTEM KARST GORONTALO, INDONESIA
Keywords:
Actinomycetes, S Aureus E.P, Aeruginosa, AntibioticsAbstract
Several pathogenic bacteria such as Staphylococcus aureus, Escherichia, Pseudomonas aeruginosa and other pathogenic bacteria have developed resistance to many marketed antibiotics. Staphylococcus aureus is already resistant to several types of antibiotics such as penicillin, erythromycin, cefoxitin, levofloxacin, and clindamycin, Escherichia is resistant to several antibiotics such as Penicillin G, Cefotaxime, Tetracycline, Ampicillin, and Amoxicillin, Pseudomonas aeruginosa has been resistant to antibiotics that target ribosomes, such as tobramycin and tigecycline. The problem of resistance of several pathogenic bacteria can be overcome by finding new metabolite compounds from Actinomycetes microorganisms that have antibiotic potential by exploring extreme areas, one of which is in the karst area, especially in plant roots. Root sampling was carried out by taking samples + 20 cm on each type of plant found. Isolation of Actinomycetes bacteria was carried out using the plate method. Antibacterial activity testing was carried out using the line method. The morphological form of the spores was seen using a SEM (Scanning Electron Microscope). The results of the study found 2 isolates of isolated plant roots containing Actinomycetes bacteria. 2 isolates showed morphological characteristics of mycelium, oval and round in shape and had various colors. The results of the antibacterial activity test showed that each isolate could inhibit several types of pathogenic bacteria, seen from the clear zone with different diameters and the results of SEM (Scanning Electron Microscopy) showed that 2 isolates had the same character and shape of spores as Actinomycetes, namely the genus Streptomyces.
References
Agustin, Alfiana Laili Dwi, and Novarina Sulsia Ista’In Ningtyas. 2022. “Resistensi Escherichia Coli Terhadap Berbagai Macam Antibiotik Pada Pasien Kucing Rumah Sakit Hewan Pendidikan Universitas Pendidikan Mandalika.” Media Kedokteran Hewan 33 (2): 63–71. https://doi.org/10.20473/mkh.v33i2.2022.63-71.
Ahmad Krisdianto, Nofiyan, and Muhammad Walid. 2023. “Gambaran Tingkat Pengetahuan Obat Antibiotik Secara Rasional Pasien Di Apotek Kimia Farma Pemalang.” Jurnal Ilmiah Multidisiplin 2 (3).
Ambarwati, C J Soegihardjo, and Langkah Sembiring. 2010. “Isolasi Dan Identifikasi Streptomycetes Dari Rizosfer Jagung (Zea Mays L.) Yang Berpotensi Sebagai Penghasil Antibiotika Isolation and Identification of Streptomycetes from Rhizosphere of Corn (Zea Mays L.) That Is Potential to Be Antibiotic Producer” 15 (1): 1–7.
Buedenbender, l., carroll, a. R., ekins, m., & kurtböke, d. I. (2017). Keanekaragaman taksonomi dan metabolit Actinomycetes yang terkait dengan tiga ascidian australia. Keragaman, 9(4). Https://doi.org/10.3390/d9040053
Jastrzębska, Ewa. 2010. "Pengaruh Bahan Kimia Perlindungan Tanaman pada Mikroorganisme yang Tinggal di Tanah." Masalah Kontemporer Manajemen dan Perlindungan Lingkungan. Jilid 5.
Matsumoto, Atsuko, dan Yoko Takahashi. 2017. "Actinomycetes Endofit: Sumber Senyawa Bioaktif Baru yang Menjanjikan." Jurnal Antibiotik. Grup Penerbitan Alam. https://doi.org/10.1038/ja.2017.20.
Nurmujahidin, Giyanto Giyanto, dan Dadang. 2023. "Pengendalian Biologis Penyakit Pembusukan Biji-bijian Bakteri yang Disebabkan oleh Burkholderia Glumae Menggunakan Actinomycetes." Jurnal Fitopatologi Indonesia 19 (2): 63–73. https://doi.org/10.14692/jfi.19.2.63-73.
Retnowati, Yuliana, Novri Youla Kandowangko, Abubakar Sidik Katili, dan Wawan Pembengo. 2024. "Keanekaragaman Actinomycetes pada Rhizosfer Tumbuhan Ekosistem Karst Gorontalo, Indonesia." Biodiversitas 25 (3): 907–15. https://doi.org/10.13057/biodiv/d250301.
Sanz-García, Fernando, Sara Hernando-Amado, dan José L. Martínez. 2018. "Evolusi mutasi resistensi Pseudomonas aeruginosa terhadap antibiotik penargetan ribosom." Perbatasan dalam Genetika 9 (OCT). https://doi.org/10.3389/fgene.2018.00451.
Schulte, Rebecca H., dan Erik Munson. 2019. "Pola Resistensi Staphylococcus Aureus di Wisconsin: Pengawasan Organisme Wisconsin 2018 untuk Tren Resistensi Antimikroba dan Laporan Program Epidemiologi (Swotare)." Kedokteran dan Penelitian Klinis 17 (3–4): 72–81. https://doi.org/10.3121/cmr.2019.1503.
Simeis, Davide De, dan Stefano Serra. 2021. "Actinomycetes: Sumber Senyawa Bioaktif yang Tidak Pernah Berakhir—Tinjauan Tentang Produksi Antibiotik." Antibiotik. MDPI AG. https://doi.org/10.3390/antibiotics10050483.
TUFEKCI, Enis Fuat, Ümit UZUN, Nagihan SAĞLAM ERTUNGA, Ayşenur BİBER, İnanç Alptuğ HIDIROĞLU, İlayda TEKKILIÇ, Birhan ALTAY, dan Ali Osman KILIÇ. 2023. "Investigasi Aktivitas Antimikroba dan Urutan 16S RRNA dari Actinomycetes yang Diisolasi dari Gua Karst di Wilayah Laut Hitam Timur Türkiye." Jurnal Pertanian dan Alam Universitas Kahramanmaraş Sütçü İmam, Mei. https://doi.org/10.18016/ksutarimdoga.vi.1226184.
Zainal Abidin, Zaima Azira, Nurfathiah Abdul Malek, Zarina Zainuddin, dan Ahmed Jalal Khan Chowdhury. 2016. "Isolasi Selektif dan Aktivitas Antagonistik Actinomycetes dari Hutan Mangrove Pahang, Malaysia." Perbatasan dalam Ilmu Hayati 9 (1): 24–31. https://doi.org/10.1080/21553769.2015.1051244.
